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KMID : 0377619630050030327
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Korean Jungang Medical Journal
1963 Volume.5 No. 3 p.327 ~ p.338
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The Effects of Anabolic Horomone on the Accelerating Mechanism of Erythrocyte Sedimetation Rate
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Abstract
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It is generally accepted that the increased rouleaux formation of erythrocytes is the main cause of the increased rate of the sedimentation and finally the increased rouleaux formation is due to the increased fibrinogen and simultaneously decreased albumin level in the plasma protein concentration.
Kuh. Han, Chae and Sohn in our laboratory confirmed that the liver took a part in the protein metabolism following injury such as burn, croton. oil-dermatitis, sup puration and tuberculosis. Chae and Sohn clarified the role of the adrenal cortex in relation with the liver on the mechanism of the acceleration of E. S. R., that is, of the changes of plasma protein fractions in the rats following acute infection by studying electrophoretic patterns of plasma protein and the attitude of mitochondria (Chae)and-RNA and DNA(Sohn)in the liver cells.
On the other hand, Whipple and his coworkers reported that the decreased albumin level and increased fibrinogen of the plasma protein in the dogs with sterile abscess by turpentine was due to the increased turn-over rate, and that the protein me taboiism was anabolic. change. According to Levenson and et al; in the study of using Nis labeled. glycine, the incorporation rate of N15 was greater in the tissue of the burned.. rats than in the tissue of the control rats, and from these results, they asserted that the anabolism of protein occurred in the burned rats.
Methods and Materials
1. Male albino-rat used in these experiments were about 200 gin. of body weight.
2. Adrenalectomy was performed via the back skin.
3. Burn was induced with boiling water on the one third of skin surface.
4. The determination of sedimentation rate, total plasma protein and fibrinogen was done by the micromethod, by tyrosin method(´MMatsuoka) and by the tryrosin method(Weichselboum) respectively.
5. The paper electrophoresis was performed by Shin modified method of Tiselius.
Part Ⅰ .
To confirm the effects of anabolic hormone on the weight gaining, on the plasma protein patterns and on the E. S. R., the determination of body weight, E. S. R. and plasma protein fractions level were performed in. the normal rats and burned rats.
The results were as follows :
1. In the burned rats, a high sedimentation rate was observed and concomitantly a slight decrease of plasma albumin, but markedly increased plasma fibrinogen were occured.
2. A marked increase of the body weight, but a-slight changes of plasma protein patterns of the control rats was induced by the administration of N. A. P. P. and T. S. P.
3. In the burned rats, N. A. P. P. and T. S. P. caused more higher sedimentation rate and more marked changes of the plasma protein patterns.
Part Ⅱ.
Chae and Sohn reported that the burn failed to to induce the acceleration of E. S. R. and change of plasma protein patterns in the adrenalectomized rats. Under this condition, the infusion of cortisone or IIOCA corrected the influence of the adrenalectomy. And in order to find whether or not the anabolic hormone could substitute the adrenal cortical hormone, these experiments were performed.
The results were as follows:
1. In the adrenalectomized rats, the anabolic hormone induced little change of E. S. R. and electrophoretic patterns of plasma protein.
2. In the adrenalectomized rats, the anbolic hormone plus burn caused little changes of E. S, R. and of electrophoretic patterns of plasma protein.
3. In the adrenalectomided rats, the infusion of cortisone and N. A. P. P. in combination induced little changs of E. S.R. and of electrophoretic patterns of plasma protein, but both cortisone plus burn caused a maked changes of E. S.. R. and of electrophoretic patterns of plasma protein.
4. In the abrenalectomized rats, the combination of the anabolic hormone with both cortisone plus burn caused more maked changes of E. S. R. and of electrophoretic patterns of plasma protein.
Part Ⅲ.
Chae observed the behavior of the mitochondria of the liver cells in relation with the changes of E. S. R. and the plasma protein patterns in the burned rats and in the adrenalectomized rats. And he reported that the mitochondria showed morphol= logical activity in parallel with the acceleration of the sedimentation. Sohn also obsserved the attitute of th RNA of the liver cells in the same procedure with Chae´s.
Since the function of the mitochondria and the amounts of the RNA of the liver cells have an intimate relation with protein metabolism, author intended to confirm the effect of anabolic hormone on the mitochondria and RNA of the liver cells in the burned rats and in the adrenalectomized rats.
In addition to, author also compared the effect of anabolic hormone with the effect of cortisone on the mitochondria and RNA of the liver cells.
The results were as follows :
1. In the normal rats, the anabolic hormone induced the proliferation of the - nuleus, the icrease of RNA in the cytoplasma of the liver cells and the multiplication of the mitochondria of the liver cells.
2. in the burned rats, the anabolic hormone caused more intensified increase of RNA and multiplication of the mitochondria of the liver cells.
3. In the adrenalectomized rats, little changes of the liver cells was observed by the administration of the anabolic hormone.
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